ABSTRACT
OBJECTIVE：To study the improvement effects of total glucosides of Paeonia l actiflora（TGPL）combined with cisplatin on tumor inhibition and renal injury in gastric cancer model rats. METHODS ：Totally 75 rats were divided into control group（normal saline ，i.g.），model group （normal saline ，i.g.），cisplatin group （25 mg/kg，i.p.），TGPL group （200 mg/kg，i.g.）， TGPL+cisplatin group （200 mg/kg TGPL ，i.g.+25 mg/kg cisplatin ，i.p.），with 15 rats in each group. Except for control group ， gastric cancer rat models were established in other groups by subcutaneous inoculation of gastric cancer BGC 823 cell suspension. After modeling ，they were given relevant medicine intraperitoneally/intragastrically ，for consecutive 28 d. The tumor weight and tumor inhibition rate were measured. The apoptosis rate in tumor tissue was detected by flow cytometry ；Western blotting assay was used to detect the levels of Bcl- 2 and Bax in tumor tissue ；ELISA or biochemical analyzer were used to determine the levels of IL-18 and KIM- 1 in urine ，BUN and Scr in serum ，as well as SOD， MDA and GSH in renal tissue ； histopathological 015）changes of renal tissue in rats were detected by HE staining. 65896961。E-mail：taolitianxia999@163.com RESULTS： Compared with control group ， there was no statistical significance in the levels of IL- 18 and KIM- 1 in urine，serum levels of BUN and Scr ，the levels of SOD MDA and GSH in renal tissue of model group （P＞0.05）；no obvious histopathological change was found in renal tissue. Compared with model group ，tumor weight in cisplatin group was decreased significantly ，while cell apoptosis was increased significantly，while expression level of Bax in tumor tissue was increased significantly ，the expression level of Bcl- 2 was decreased significantly（P＜0.05）；the levels of IL- 18 and KIM- 1 in urine ，BUN and Scr in serum and MDA in kidney were increased significantly（P＜0.05）；the levels of SOD and GSH in renal tissue were decreased significantly （P＜0.05）；renal tubules were dilated，vacuoles of epithelial cells in basal layer and cast in renal tubules formed . Compared with cisplatin ，tumor weight in TGPL+cisplatin group was decreased significantly ，while apoptotic rate was increased significantly ，while the level of Bax was increased significantly ，expression level of Bcl- 2 was decreased significantly （P＜0.05）；the levels of IL- 18 and KIM- 1 in urine ， BUN and Scr in serum and MDA in renal tissue were significantly reduced （P＜0.05）；the levels of SOD and GSH in renal tissue were increased significantly （P＜0.05）；there was no tubule dilation ，but vacuoles were occasionally found in basal epithelial cells，and the cast formation in renal tubules was rare. Compared with TGPL group，tumor weight in TGPL +cisplatin group was significantly reduced ，the tumor inhibition rate was significantly increased ，the apoptosis rate was significantly increased ，while the expression level of Bax in tumor tissue was increased significantly ，expression level of Bcl- 2 was decreased significantly （P＜ 0.05）；there was no statistical significance in the levels of IL- 18 and KIM- 1 in urine ，the levels of BUN and Scr in serum ，the levels of SOD ，MDA and GSH in renal tissue （P＞0.05）；a small amount of inflammatory cells infiltrated occasionally in renal tissue. CONCLUSIONS ：TGPL combined with cisplatin can inhibit tumor growth ，promote tumor cell apoptosis and improve renal injury induced by cisplatin.